CYAO Project CYAO: Cyanobacterial Platform Optimised for Bioproduction
Photosynthesis Research Unit The Institute of Biophysics is an internationally renowned research institute, part of the Department of Physical Sciences and Material Technologies of the National Research Council of Italy (CNR). The photosynthesis research unit at the Milan UOS focuses on biochemical, biophysical and physiological investigations of the photosynthetic apparatus. This unit has more than three decades of internationally recognised research in the field, having been the pioneer of these studies in Italy and one of the leading laboratory in Europe since the early sixties. In the past decades attention was dedicated to the study of microalgae and infrastructures required for their growth, such as light-controlled incubators, sterile rooms, cell counters, and different microscopes are present. Spectroscopy is a core research topic and a broad range techniques are available: high-resolution absorption, fluorescence, double-beam/double-wavelength spectrometers and circular-dichroism as well as fluorescence lifetime (picosecond), absorption transient (microsecond), fluorescence inductions (micro-to-millisecond) and pulse amplitude modulated instruments (time resolved). All the measurements can be performed in the 400 to 4.2 K temperature range. A complement of biochemical tools, including, electrophoresis, FPLCs, HPLCs, a battery of fully equipped ultracentrifuge and tools for basic molecular biology practise are also present.
Contact: Stefano Local & Project Coordinator
IBBA Milan The Institute of Agricultural Biology and Biotechnology is part of the Bio-Agrifood Department of the National Research Council. It comprises four research centres (UOS) with 40 researchers. The Institute main goal is the development of fundamental knowledge, a prerequisite for the understanding and valorisation of biodiversity as well as for all genetic, technological and biotechnological applications in the agricultural field. Research activities comprise the development of technologies and biotechnologies of agrarian and industrial interest, including molecular techniques for gene sequence engineering suited to the production of metabolites and proteins and the improvement of non-food plants for biofuel production. General biochemistry, microbiology (Biosafety Level 1), microscopy (Zeiss Axiovert 200 and Apotome system) and molecular and cellular laboratories, plant growth facilities, a sequencing and bioinformatics centre are present.
Website: IBBA, Milan
Contact: Paola Local Coordinator
ISE Verbania The Institute for Ecosystem Study carries out studies on ecosystem structure and dynamics and on its response to anthropogenic disturbance and develops methods of environmental monitoring. Since 1938, the main section in Verbania carries out studies on lakes in Italy and in the World, investigating structures, functions and fluctuations of lake ecosystems in their natural state or as a response to anthropogenic pressures, including climate change. Expertise covers all aspects of limnology, from community ecology, hydromorphology, chemistry to plankton, macrophytes, benthos and fish ecology. The CNR-ISE is involved in many projects of lake restoration. It has been involved in the application in Italy of the Water Framework Directive, as an official consultant of the Italian Ministry for Environment: this role has been fulfilled by developing methods for lake monitoring and biotic indexes for ecological assessment. ISE team can exploit various lab facilities: climatic chambers, light sources, microscopes, autoclaves, HPLC, spectrophotometer; a complete limnological instrumentation for field sampling is also available to collect chemical and biological samples. CNR-ISE has also renovated its historical fish hatchery. The plant is seminatural including two large outside pools for fish rearing and two rooms for eggs hatching and fish breeding in dedicated tanks.
Website: ISE, Verbania
Contact: Andrea, Pietro or Nicoletta Local Coordinators
Centre Algatech Trebon CZ The Institute of Microbiolgy of the Academy of Sciences of the Czech Republic has three satellite working units, the first of which is The Algatech Centre located in Trebon. It has been internationally recognised for more than 50 years for its research on photosynthetic microorganisms, algae, cyanobacteria and photosynthetic bacteria from molecular/cellular to eco-physiological levels, as well as for the development of new bio-optical instruments and technology for the cultivation of algae and algal products. State of art facilities include specific equipments and instrumentations (algal growth facilities, photobioreactors, biochemistry lab., confocal microscopy), standard spectrometers, several kinetic fluorometers. Other core topics are the phototrophic cultivations of microalgae including prokaryotic cyanobacteria, cultivation of selected freshwater strains for biomass production and isolation of bioactive compounds, design and construction of cultivation systems and photobioreactors. Laboratory photobioreactors and large-scale outdoor units are available for the production of microalgae biomass. Laboratory set-up and full biotechnology equipment allows cultivation, analysis and complete processing of biomass (separation - disintegration - drying), fractionation and characterisation, including analysis and identification of selected valuable substances in (carotenoids, lipids, polyunsaturated fatty acids, polysaccharides) using high performance liquid chromatography and gas chromatography with mass spectrometry (HPLC-MS, GC-MS).
Website: Centre Alagetch, Trebon, CZ
Contat: Ondrej Prasil Local Coordinator and Director of the Centre
The research is founded by the Cariplo Foundation under the Biotechnology founding scheme.
Fondazione Cariplo
The site is under constructure. CYAO-Web.V2. 20/05/2018